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Chordate gateway vector set

Laurent chabry's work

Novembre 6th, 2007: The double cassette destinations vectors pSP72BSSPE-R3- ccdB/cmR-R5::RfA-HA, pSP72BSSPE-R3- ccdB/cmR-R5::HA-RfA, pSP72BSSPE-R3- ccdB/cmR-R5::RfA-CFP (respectively D9, E9 and F9 from the Gateway Collection Plate Release May 2007) have now been tested in vivo. An immunostaining was performed on embryos to reveal the fusion proteins resulting from the recombination of the double cassette destination vectors (D9, E9 or F9) with 2 entry clones (a promoter and a transcription factor ORF). We observed fluorescence in the expected embryonic territories. All three constructs are thus functional. Good luck and have fun with these tools.

October 30, 2007: Dear Gateway users, A new version of the Chordate Gateway Manual has been made available. You can download it on the following link V6.7-Chordate_Gateway_Manual.pdf (.pdf, 414Kb)

May 16th, 2007: A detailed Gateway protocol is now availabe. You can download it by clicking the following link Detailed Gateway protocol.pdf (.pdf, 160Kb)

-- Agnes Roure